FIG. 5.

Cox1 assembly intermediates in cells compromised in heme a synthesis. (A) Lysates from wild-type (WT) or cox10Δ mitochondria with genomically tagged Mss51-Myc, Coa1-Myc, or Shy1-Myc were subjected to BN-PAGE, and the distribution of the respective complexes was analyzed by immunoblotting as described for Fig. 1. (B) The steady-state levels of Shy1-Myc were assessed as described for Fig. 1. (C) In vivo labeling of mitochondrial translation products in SHY1::Myc cox10Δ cells expressing either empty vector or COX10 or overexpressing MSS51. Cells were treated and analyzed as for Fig. 2D. (D) Distribution of Shy1-Myc complexes in SHY1::Myc cox10Δ cells carrying either COX10 or empty vector or overexpressing MSS51, analyzed by native electrophoresis.