FIG. 1.

Mutation of a CRE site in the FSHβ promoter results in loss of preferential GnRH stimulation at low pulse frequencies. LβT2 cells were transfected with (A) wild-type −140/+15 rFSHβLuc reporter or (B) mutant CRE −140/+15 rFSHβLuc reporter. The cells were stimulated with pulsatile GnRH at a low (∧__∧; one pulse/120 min) or high (∧∧∧; one pulse/30 min) frequency. Bar graphs show means ± the standard errors of the mean (SEM) fold stimulation for each experimental condition. The data presented are a pool of four to five independent experiments, each performed in triplicate. Significant differences (P < 0.05), measured by one-way analysis of variance (ANOVA) with a post hoc Tukey-Kramer multiple-comparison test, are indicated by different letters.