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. 2009 Dec 14;30(4):985–994. doi: 10.1128/MCB.00909-09

FIG. 3.

FIG. 3.

The Ura3-Pcl5 processing product is not inherently unstable. (A) Characterization of the Ura3-Pcl5 processing product size. Wild-type cells expressing Ura3 fused to various fragments of Pcl5 from plasmids KB1908 (1-46), KB1909 (1-53), KB1910 (1-65), or hul5Δ mutant cells expressing full-length Ura3-Pcl5 (F.L.) were pulse-labeled for 10 min and then subjected to immunoprecipitation and SDS-PAGE. (B) Ura3-Pcl5(1-53) expressed from KB1909 in wild-type and hul5Δ cells was subjected to stability analysis as for Fig. 1A.