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. 2009 Dec 7;30(4):1049–1058. doi: 10.1128/MCB.01344-09

FIG. 3.

FIG. 3.

Atg13-8SA promotes Atg1 complex formation, Atg1 activation, and PAS organization. (A) Formation of the Atg1 complex. Cells (YYK412) harboring the indicated p416GAL1[ATG13] plasmid grown in SCRaf were incubated in SCRafGal for 2 h to induce Atg13 protein. Atg13 and Atg17 coimmunoprecipitated with in HAAtg1 were detected by immunoblotting. The catalytic activity of Atg1 kinase was also examined using myelin basic protein (MBP) as a substrate. The asterisk denotes a nonspecific band in the total cell lysates that was recognized by the anti-HA ascites. α, anti. (B) PAS assembly of Atg proteins. Cells (TMK625, atg11Δ) grown in SCRaf were shifted to SCRafGal for 1 h to induce Atg13. GFP-Atg17 and mCherry-Atg8 were observed by fluorescence microscopy.