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. 2009 Dec 7;30(4):1049–1058. doi: 10.1128/MCB.01344-09

FIG. 5.

FIG. 5.

Expression of Atg13-8SA mimics TORC1 inactivation to induce autophagy. (A) Cells grown in SCRaf were treated with 0.2 μg/ml rapamycin for 4 h, incubated in SCRafGal for 4 h, or incubated in SCRafGal for 2 h before a 2-h treatment with 0.2 μg/ml rapamycin (Rapa). Atg1 complex formation and Atg1 kinase activity were examined as for Fig. 3A. α, anti. (B) Pho8Δ60-expressing cells harboring the indicated p306GAL1[ATG13] plasmids were treated as for panel A and subjected to the ALP assay. Error bars represent the SD from three independent experiments.