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. 2009 Dec 7;30(4):1049–1058. doi: 10.1128/MCB.01344-09

FIG. 6.

FIG. 6.

Autophagy induction by Atg13 constructs. (A) Pho8Δ60-expressing cells harboring the indicated p416GAL1[ATG13] plasmids were subjected to the ALP assay before or after a 4-h incubation with SCRafGal. Error bars represent the SD from at least three independent experiments. (B) Atg13 proteins were detected by immunoblotting. (C) Summary of in vitro TORC1 assay and ALP assay using various Atg13 constructs. The shaded box represents the Atg1-binding site.