FIG. 7.

Effect of Atg13-8SA on other Atg proteins. (A) Atg13-8SA expression does not significantly affect Atg protein expression. Atg protein levels in cells used for panel A were assessed by immunoblotting. The asterisk denotes the phosphatidylethanolamine-conjugated form of Atg8. (B) Requirement for ATG genes for autophagy induced by Atg13-8SA expression. Cells harboring the indicated mutations carrying the p416GAL1[ATG13-8SA] plasmid were subjected to the ALP assay before or after a 4-h incubation with SCRafGal to induce Atg13-8SA expression. Error bars represent the SD form three independent experiments.