TABLE 1.
Yeast strains used in this study
| Strain name | Genotype | Source or reference |
|---|---|---|
| W303-1B | MATaade2 his3 leu2 trp1 ura3 can1 | Laboratory stock |
| BJ2168 | MATaleu2 trp1 ura3 pep4-3 prb1-1122 prc1-407 | Laboratory stock |
| BY4741 | MATahis3 leu2 met15 ura3 | Laboratory stock |
| OND88 | W303-1B pho8::TDH3p::pho8Δ60 | 20 |
| YYK742 | OND88 atg17Δ::KanMX | This study |
| YYK744 | OND88 atg29Δ::KanMX | This study |
| YYK757 | OND88 atg2Δ::KanMX | This study |
| YYK762 | OND88 atg11Δ::LEU2 | 20 |
| YYK797 | OND88 atg1Δ::URA3 | This study |
| YYK334 | W303-1B tor1Δ::KanMX | This study |
| YYK798 | YYK334 pRS313[HA-TOR1] pRS316vector | This study |
| YYK799 | YYK334 pRS313[HA-TOR1] pRS316[Flag-KOG1] | This study |
| YYK800 | YYK334 pRS313[HA-tor1-D2294E] pRS316[Flag-KOG1] | This study |
| YYK412 | BJ2168 HA-ATG1 | 15 |
| TMK625 | BY4741 atg11Δ::LEU2 ATG17-GFP::KanMX | 20 |