TABLE 2.
Plasmids used in this study
| Plasmid name | Description | Source or reference |
|---|---|---|
| YEp352 | URA3 2μm ARS Ampr | Laboratory stock |
| p416GAL1 | URA3 CEN6-ARSH4 AmprGAL1 promoter | Laboratory stock |
| pRS313 | HIS3 CEN6-ARSH4 Ampr | Laboratory stock |
| pRS314 | TRP1 CEN6-ARSH4 Ampr | Laboratory stock |
| pRS316 | URA3 CEN6-ARSH4 Ampr | Laboratory stock |
| YEp352[ATG13] | YEp352 ATG13 | 5 |
| YEp352[ATG13-8SA] | YEp352 ATG13 S348A S437A S438A S496A S535A S541A S646A S649A | This study |
| p416GAL1[ATG13] | p416GAL1 ATG13orf | This study |
| p416GAL1[ATG13-2SA] | p416GAL1 ATG13orf S348A S496A | This study |
| p416GAL1[ATG13-3SAa] | p416GAL1 ATG13orf S437A S438A S496A | This study |
| p416GAL1[ATG13-3SAb] | p416GAL1 ATG13orf S496A S535A S541A | This study |
| p416GAL1[ATG13-3SAc] | p416GAL1 ATG13orf S496A S646A S649A | This study |
| p416GAL1[ATG13-4SAa] | p416GAL1 ATG13orf S437A S438A S646A S649A | This study |
| p416GAL1[ATG13-4SAb] | p416GAL1 ATG13orf S348A S496A S535A S541A | This study |
| p416GAL1[ATG13-5SA] | p416GAL1 ATG13orf S437A S438A S496A S646A S649A | This study |
| p416GAL1[ATG13-6SA] | p416GAL1 ATG13orf S348A S437A S438A S496A S646A S649A | This study |
| p416GAL1[ATG13-7SAa] | p416GAL1 ATG13orf S437A S438A S496A S535A S541A S646A S649A | This study |
| p416GAL1[ATG13-7SAb] | p416GAL1 ATG13orf S348A S437A S438A S535A S541A S646A S649A | This study |
| p416GAL1[ATG13-8SA] | p416GAL1 ATG13-8SAorf | This study |
| pRS313[HATOR1] | pRS313 3×HA-TOR1 | 30 |
| pRS313[HATOR1-KD] | pRS313 3×HA-TOR1-D2294E | 30 |
| pRS316[KOG1] | pRS316 KOG1 | 30 |
| pRS316[FlagKOG1] | pRS316 Flag-KOG1 | 30 |
| pRS316[ATG1] | pRS316 ATG1 | 28 |
| pRS316[atg1D211A] | pRS316 atg1-D211A | 28 |
| pRS314[atg1K54A] | pRS316 atg1-K54A | 19 |