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. 2009 Dec 2;48(2):412–418. doi: 10.1128/JCM.01315-09

TABLE 1.

MLVA automated genotyping primers

Primer Sequence (5′-3′)a Final concn (nM)
Multiplex 1
    CDR5F VIC-AATTTTAAGTTAACGTTTTTCTACAT 400
    CDR5R GTGTCTTAGCCATTTTTATCAATCCTTTCTAT 400
    CDR48F VIC-AGGAGCTTTATATGGACATTCAGGTAG 300
    CDR48R GTGTCTTAATCTCTTTCAAACTCTTCAATCTCAAT 300
    CDR49F PET-AACATATTTAGGCATTTTAGTC 200
    CDR49R GTGTCTTGAGTATTATTTATCATTTGTGGGTATTA 200
    CDR60F NED-GGTGCACATGCTGGTCCTG 100
    CDR60R GTGTCTTAACGCATTAAATTTCACTCCTCATAC 100
Multiplex 2
    CdA6F NED-TTAATTGAGGGAGAATGTTAAA 50
    CdA6R GTGTCTTAAATACTTTTCCCACTTTCATAA 50
    CdG8F VIC-TGTATGAAGCAAGCTTTTTATT 100
    CdG8R-2 GTGTCTTCACCAAAAATTTCTAACCAA 100
a

Boldface type indicates seven-nucleotide “pigtails.”