TABLE 1.
Primer | Sequence (5′-3′)a | Final concn (nM) |
---|---|---|
Multiplex 1 | ||
CDR5F | VIC-AATTTTAAGTTAACGTTTTTCTACAT | 400 |
CDR5R | GTGTCTTAGCCATTTTTATCAATCCTTTCTAT | 400 |
CDR48F | VIC-AGGAGCTTTATATGGACATTCAGGTAG | 300 |
CDR48R | GTGTCTTAATCTCTTTCAAACTCTTCAATCTCAAT | 300 |
CDR49F | PET-AACATATTTAGGCATTTTAGTC | 200 |
CDR49R | GTGTCTTGAGTATTATTTATCATTTGTGGGTATTA | 200 |
CDR60F | NED-GGTGCACATGCTGGTCCTG | 100 |
CDR60R | GTGTCTTAACGCATTAAATTTCACTCCTCATAC | 100 |
Multiplex 2 | ||
CdA6F | NED-TTAATTGAGGGAGAATGTTAAA | 50 |
CdA6R | GTGTCTTAAATACTTTTCCCACTTTCATAA | 50 |
CdG8F | VIC-TGTATGAAGCAAGCTTTTTATT | 100 |
CdG8R-2 | GTGTCTTCACCAAAAATTTCTAACCAA | 100 |
Boldface type indicates seven-nucleotide “pigtails.”