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. 2009 Dec 2;48(2):503–511. doi: 10.1128/JCM.01761-09

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FIG. 2. — RLB results for 16 Nocardia isolates representing 14 species. Lane 1, isolate ATCC BAA-279^T (N. abscessus); lane 2, isolate ATCC 19247^T (N. asteroides); lane 3, isolate 96-247-0894 (N. blacklockiae); lane 4, isolate ATCC 19296 (N. brasiliensis); lane 5, isolate ATCC 15333^T (N. brevicatena); lane 6, isolate ATCC 6847 (N. carnea); lane 7, isolate 00-159-1584 (N. cyriacigeorgica); lane 8, isolate ATCC 33726^T (N. nova); lane 9, isolate ATCC 14629^T (N. otitidiscaviarum); lane 10, isolate 00-355-2649 (N. thailandica); lane 11, ATCC 6865^T (N. transvalensis); lane 12, isolate ATCC BAA-509 (N. veterana); lane 13, isolate ATCC BAA-278^T (N. paucivorans); lanes 14 to 16, isolates ATCC 3308, 05-048-3600, and 07-023-0418 (all N. farcinica isolates). The left column shows the probe list. The blot shows (i) the taxon-specific probes ACTMY-ITS-a and ACTMY-ITS-b to flag all isolates as possible Nocardia spp., (ii) the group-specific probe Nfneg-16S hybridizing with all Nocardia species tested other than N. farcinica, (iii) the group-specific probe Ncyr/far-16S hybridizing with both N. cyriacigeorgica and N. farcinica, (iv) weak cross-reaction between probe Nbra-16S and DNA from N. thailandica, and (v) different N. farcinica isolates hybridizing with either or both of the Nfar-ITS-a and Nfar-ITS-b probes.