TABLE 1.

Primer and probe sequences

Reagent	Sequencec	Reference or source
Primersa
Pan-species forward	GTT AAG GGA GTG AAG ACG ATC AGA TA	38
Pan-species reverse	AAC CCA AAG ACT TTG ATT TCT CAT AAG	38
P. falciparum 18S rDNA forward	ATT GCT TTT GAG AGG TTT TGT TAC TTT	46
P. falciparum 18S rDNA reverse	GCT GTA GTA TTC AAA CAC AAT GAA CTC AA	46
P. ovale 18S rDNA forward	CCG ACT AGG TTT TGG ATG AAA GAT TTT T	39
P. ovale 18S rDNA reverse	CAA CCC AAA GAC TTT GAT TTC TCA TAA	46
P. malariae 18S rDNA forward	AGT TAA GGG AGT GAA GAC GAT CAG A	46
P. malariae 18S rDNA reverse	CAA CCC AAA GAC TTT GAT TTC TCA TAA	46
Human GAPDH forward	CCT CCC GCT TCG CTC TCT	This study
Human GAPDH reverse	GCT GGC GAC GCA AAA GA	This study
P. falciparum LDH forward	ACG ATT TGG CTG GAG CAG AT	36
P. falciparum LDH reverse	TCT CTA TTC CAT TCT TTG TCA CTC TTT C	36
MGB probesb
Pan-species	VIC-TCG TAA TCT TAA CCA TAA AC	38
P. falciparum	FAM-CAT AAC AGA CGG GTA GTC AT	46
P. ovale	VIC-CGA AAG GAA TTT TCT TAT T	38
P. malariae	FAM-ATG AGT GTT TCT TTT AGA TAG C	46
Human GAPDH	VIC-CCT CCT GTT CGA CAG TCA GCC GC	This study
TaqMan probeb (P. falciparum LDH)	FAM-GTA ATA GTA ACA GCT GGA TTT ACC AAG GCC CCA-TAMRA	36

^aSynthesized by MWG/Operon Biotech (High Point, NC) and resuspended in molecular-grade water. LDH, lactate dehydrogenase.

^bSynthesized by Applied Biosystems (Foster City, CA) and diluted in Tris-EDTA Buffer (FisherBioTech, Fair Lawn, NJ).

^c“FAM” and “VIC” denote fluorescent dyes.