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. 2009 Dec 2;48(2):581–585. doi: 10.1128/JCM.00543-09

TABLE 2.

Details of gene loci and corresponding primer sequences used for MLSA

Gene	Product	Locus^a	Gene size (bp)	Coordinates	Size of analyzed polymorphic sequence (bp)	G+C content (%)	No. of alleles	Primer sequence (5′-3′)^b
accA2	Acetyl coenzyme A	LIC11517	1,667	1874272-1875939	452	41.59	23	`+ TTGATGCTTATGTTTGGGTTCAAT`
	carboxylase α subunit							`− AGAATCGAATAAATCTAGTTCCT`
ccmF	Cytochrome c biogenesis	LIC10813	2,189	983052-985241	295	40.31	17	`+ GATATTCCTGTACAATTCCGG`
	factor							`− GGCAGTTTTCTTTTTAATAAC`
czcA	Heavy-metal efflux pump	LIC11937	3,320	2340958-2344278	448	41.75	15	`+ TCTTTTGGGAGAAATGATCGG`
								`− TAAGTTTGAAAGACTACAACG`
gcvP	Glycine cleavage system P	LIC10309	2,894	350674-353568	275	41.28	14	`+ AAGAGCTAAAAGATCTGCAGC`
	protein							`− CTGGCCTTGAAATTTCGAACG`
groEL	60-kDa chaperonin (Hsp60)	LIC11335	1,640	1646985-1648625	553	42.2	26	`+ TCGCCTCGTCTCCATCCA`
								`− TGACGCAGAATCCATGGTAG`
polA	DNA polymerase I	LIC10586	2,753	711562-714315	432	38.11	12	`+ TCATAGTCGTTTGAATCTACGGTAAAA`
								`− ATTAAAGATTCCGGTGTAGAACCAA`
recF	DNA replication and repair	LIC10003	1,097	5062-6159	400	31.94	22	`+ CGGCGGAAAGAAGAAAGTT`
	protein RecF							`− CTCGACTTTTCCTTGTCGAA`

^a

L. interrogans serovar Copenhageni strain Fiocruz L1-130 has been used as a reference.

^b

+, foward primer; −, reverse primer.