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SDS–PAGE shows the two major steps in the purification of P. somniferum SalR. Lane 1, molecular-weight markers; lane 2, crude E. coli extract; lane 3, flowthrough of Talon affinity resin; lane 4, eluate of the affinity resin; lane 5, eluate of gel filtration after thrombin digestion; lane 6, molecular-weight markers. The arrow indicates untagged SalR.
