Figure 7. Enhanced specific cytotoxic activity in PBMC co-cultivated with LB1319-MEL cells pre-treated with hIFN-γ+GGTI-298.

Cytotoxic activities of in vitro stimulated PBMC were tested after two stimulations with LB1319-MEL cells either untreated or pretreated with hIFN-γ (50 IU/mL); GGTI-298 (10 µM); or hIFN-γ (50 IU/mL) plus GGTI-298 (10 µM). A) Cytotoxicity was evaluated by CD107a expression on CD8+ gated T lymphocytes. Cytotoxic activity was also tested on an unspecific target cell: B) Jurkat and the specific one: C) LB1319-MEL. Experiments were performed in triplicate with PBMC from one healthy donor representative of one other giving comparable results.