Skip to main content
. 2010 Feb 3;5(2):e9043. doi: 10.1371/journal.pone.0009043

Figure 8. PBMC stimulation with hIFN-γ+GGTI-298 pretreated LB1319-MEL cells induces specific cells proliferation, labeled with MART-1/HLA-A2 tetramers.

Figure 8

A) HLA-A0201 positive PBMC from one representative HD were stimulated twice in vitro with LB1319-MEL cells either untreated (NT) or pretreated with 50 UI/mL hIFN-γ (IFN), or 10 µM GGTI-298 (GGTI), or with both hIFN-γ and GGTI-298 (IFN+GGTI). At the end of the culture period, specific TCR MelanA / HLA-A2 expression was evaluated on CD8+ gated T lymphocytes. B) HLA-A0201 positive PBMC from the same HD were stimulated twice in vitro with LB1319-MEL cells either untreated (NT) or pretreated with 50 UI/mL hIFN-γ (IFN), or 10 µM GGTI-298 (GGTI), or with both hIFN-γ and GGTI-298 (IFN+GGTI) or with irradiated (HLA-A0201) L1-EBV cells. At the end of the culture period, specific TCR EBV/HLA-A2 expression was evaluated on CD8+ gated T lymphocytes.