Table 1. Enhanced IFN-γ secretion by PBMC co-cultivated with LB1319-MEL cells pre-treated in vitro with hIFN-γ+GGTI-298.

	Cytokines concentration (+/- SD)
	IL-2	IFN-γ	IL-4	IL-10
NT	1,5 (+−/1)	71,1 (+/−5)	5,98 (+/−3)	37,6 (+/−6)
IFN	3,9 (+/−2)	206 (+/−24)	42,7 (+/−3)	38,8 (+/−2)
GGTI	1,7 (+/−1)	148 (+/−19)	18,3 (+/−6)	39,3 (+/−7)
IFN+GGTI	4,2 (+/−1)	418 (+/−4)	36,3 (+/−1)	37,7 (+/−7)

PBMC from 3 HLA-A0201 HD were stimulated twice in vitro with LB1319-MEL cells untreated (NT); or pretreated with 50 UI/mL hIFN-γ (IFN); 10 µM GGTI-298 (GGTI); or the combination of 50 UI/mL hIFN-γ and 10 µM GGTI-298 (IFN+GGTI) as indicated. 24h after the second in vitro stimulation, cytokine secretions in the culture supernatants were measured by Multi-Plex human cytokine assays. Results are illustrated in pg/mL for secretions of IFN-γ, IL-2, IL-4 and IL-10. Data are shown as mean (+/− standard deviation) of duplicate values and were obtained from one donor representative of the two others giving comparable results.