Table II.

Amino acid amounts in whole plantlets and released in the growth medium

Plants were grown for 6 d in modified MS medium containing no ammonium. Amino acids were quantitated by HPLC 3 d after transfer into fresh MS medium without ammonium. Means ± se of four biological replicates are shown. Significant differences from the wild type (t test) are as follows: * P < 0.05, ** P < 0.01.

Amino Acid	Plant				Medium
	Wild Type		gdu1-1D		Wild Type		gdu1-1D
	Amounta	Percentage	Amounta	Percentage	Amounta	Percentage	Amounta	Percentage
Asp	1,699 ± 188	9.6	1,735 ± 272	10.8	41.9 ± 15.4	13.6	76.1 ± 15.8	4.1
Glu	5,024 ± 637	28.3	4,860 ± 811	30.1	39.4 ± 18.2	12.8	119.7 ± 28.2	6.5
Asn	776 ± 100	4.4	660 ± 109	4.1	15.6 ± 9.4	5.1	210.3 ± 36.8*	11.4
Ser	2,030 ± 176	11.4	1,970 ± 273	12.2	13 ± 3.7	4.2	95.7 ± 17.4*	5.2
Gln	2,174 ± 364	12.3	2,031 ± 295	12.6	72.3 ± 44.4	23.5	939.2 ± 114.8**	50.7
Gly	1,720 ± 647	9.7	1,696 ± 481	10.5	17.9 ± 4.9	5.8	62.8 ± 10.6	3.4
His	91 ± 8	0.5	80 ± 14	0.5	5.1 ± 2.2	1.7	58.9 ± 11.2*	3.2
Thr	149 ± 40	0.8	80 ± 27	0.5	41.6 ± 17.5	13.5	25.2 ± 9.3	1.4
Ala	2,738 ± 331	15.4	2,164 ± 399	13.4	47.1 ± 16.5	15.3	105.3 ± 27	5.7
Pro	407 ± 52	2.3	284 ± 73	1.8	4.8 ± 1.1	1.5	36.8 ± 7.2	2
Tyr	259 ± 56	1.5	185 ± 21	1.1	0.2 ± 0.1	0.1	4.2 ± 0.8*	0.2
Val	265 ± 70	1.5	170 ± 30	1.1	6.2 ± 2.2	2	68.3 ± 9*	3.7
Met	13 ± 2	0.1	7 ± 3	0	0 ± 0	0	3 ± 0.6*	0.2
Ile	109 ± 42	0.6	45 ± 10	0.3	0.8 ± 0.6	0.3	16.9 ± 4.1	0.9
Lys	68 ± 25	0.4	37 ± 9	0.2	0.2 ± 0.1	0.1	5.6 ± 2.1	0.3
Leu	128 ± 51	0.7	62 ± 14	0.4	0.8 ± 0.3	0.3	12.8 ± 3	0.7
Phe	88 ± 20	0.5	58 ± 12	0.4	0.8 ± 0.4	0.3	10.2 ± 1.7*	0.6
Total	17,738 ± 1,583	100	16,125 ± 2,266	100	307.6 ± 125	100	1,851.1 ± 278.9*	100

^anmol mg⁻¹ chlorophyll.