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. 2010 Feb;152(2):670–684. doi: 10.1104/pp.109.148247

Figure 3.

Figure 3.

Expression of rapeseed microsomal LPAATs. Total RNA was purified from various rapeseed tissues (A) and from developing embryos (B) at various days after pollination (DAP), transcribed in the presence (+) of reverse transcriptase (RT), and the resulting cDNA was used as a template for PCR with primers designed to discriminate between BAT1.13 and BAT1.5 transcripts. − indicates the absence of reverse transcriptase. RPL2 represents a control for RNA loading. M corresponds to the DNA size ladder added to the 49 DAP(−) lane. DAF, Days after flowering.