Figure 5.

Expression of BAT1.13 and BAT1.5 cDNAs in transgenic Arabidopsis seeds. Total RNA was purified from mature seeds, reverse transcribed, and the resulting cDNA was used as a template for PCR with primers designed to discriminate the BAT1.13 and BAT1.5 transcripts from that of the endogenous Arabidopsis gene. The resulting reaction mix was migrated on agarose gels and transferred to a nylon membrane. The membrane was hybridized sequentially with a BAT1.13 or a ³²P-labeled cDNA followed by a RPL2 ³²P-labeled probe, and the radioactive signals (A) were integrated using a phosphor imager (B).