Figure 3.

Transient expression of CaLOX1 and cell death in pepper leaves infiltrated with Agrobacterium GV3101 carrying the 35S:00 or 35S:CaLOX1 construct. A, Visible and UV light-illuminated phenotypes of the transient expression in leaves 3 d after agroinfiltration with different bacterial concentrations (OD₆₀₀). B, Immunoblotting assay of the Agrobacterium-mediated transient expression of CaLOX1 in pepper leaves. C, Staining with trypan blue and DAB of transient expression in leaf tissues 1 d after agroinfiltration. Bars = 0.2 mm. D, Ion leakage from transiently expressing leaves at different time points after agroinfiltration. E and F, Induction of CaLOX1, CaBPR1, and CaDEF1 as measured by real-time RT-PCR. The data of the relative transcript levels are normalized to the expression of the 18S rRNA gene as an internal control. Values are presented as means ± sd. Asterisks indicate significant differences between the 35S:CaLOX1 and 35S:00 constructs (Student's t test, P < 0.05). H, Healthy leaves; hai, hours after infiltration.