Table II.
List of genes with the largest changes in gene expression in aneuploid plants
Normalized microarray signals are shown for DpDf and wild-type plants for meristem and total seedling tissues.
| Accession | DpDf Meristem | Wild-Type Meristem | Fold Change (DpDf/Wild-Type Meristem)a | DpDf Seedlings | Wild-Type Seedlings | Fold Change (DpDf/Wild-Type Seedlings)ab | Chromosomec | Annotation |
|---|---|---|---|---|---|---|---|---|
| Genes with higher transcript levels in both DpDf tissues | ||||||||
| AW289130 | 537 | 60 | 8.94* | 414 | 18 | 23.09* | 5 | Cyclophilin |
| BM380426 | 1,033 | 146 | 7.09 | 339 | 31 | 11.10* | 6 | Unknown |
| BM379473 | 1,790 | 764 | 2.34 | 262 | 140 | 1.88 | 5 | Unknown |
| BM269210 | 804 | 351 | 2.29 | 198 | 81 | 0.41 | 5 | Splicing factor Prp18 |
| AI783234 | 1,424 | 656 | 2.17 | 429 | 169 | 2.53 | 5 | Aminoalcoholphosphotransferase |
| BF729248 | 846 | 422 | 2.00 | 544 | 327 | 1.66 | 1 | Unknown |
| AY105653 | 2,273 | 1,199 | 1.90 | 842 | 358 | 2.35 | 5 | Malate oxidoreductase |
| CK369759 | 298 | 172 | 1.74 | 145 | 63 | 2.32* | 5 | Unknown |
| AY107589 | 2,918 | 1,809 | 1.61 | 633 | 317 | 2.00 | 5 | Unknown |
| Genes with higher transcript levels in DpDf meristem | ||||||||
| CF626580 | 927 | 451 | 2.06 | 123 | 110 | 1.12 | 4 | Unknown |
| Genes with higher transcript levels in DpDf seedlings | ||||||||
| L16798 | 105 | 105 | 1.00 | 140 | 12 | 0.09* | 5 | Class I acidic chitinase |
| AY639019 | 384 | 293 | 1.31 | 119 | 49 | 2.41* | 5 | Phosphate transport protein |
| U17897 | 1,242 | 845 | 1.47 | 415 | 200 | 2.07 | 5 | Starch-branching enzyme I (sbe1) |
| CF629635 | 542 | 489 | 1.11 | 375 | 187 | 2.01 | 5 | Sec14-like protein |
| Genes with lower transcript levels in both DpDf tissues | ||||||||
| AY109249 | 1,122 | 2,814 | 0.40 | 242 | 571 | 0.42 | 2 | Unknown |
| AI677337 | 615 | 1,217 | 0.51 | 427 | 874 | 0.49 | 6 | Unknown |
| AI622092 | 399 | 714 | 0.56 | 92 | 200 | 0.46 | 6 | Expressed protein |
| AI737202 | 407 | 711 | 0.57 | 76 | 192 | 0.40 | 6 | Unknown |
| BM073880 | 456 | 764 | 0.60 | 48 | 109 | 0.44* | ND | GCN5L1 family protein |
| BM379784 | 375 | 608 | 0.62 | 119 | 237 | 0.50 | 6 | Unknown |
| AY107007 | 280 | 419 | 0.67 | 37 | 78 | 0.47* | 6 | Unknown |
| BM079913 | 120 | 179 | 0.67 | 62 | 135 | 0.46* | 1 | Protease inhibitor/lipid transfer |
| Genes with lower transcript levels in DpDf meristem | ||||||||
| 11990232-113 | 3,351 | 12,121 | 0.28 | 72 | 162 | 0.45 | 5 | Unknown |
| 11990232-89 | 96 | 231 | 0.41 | 49 | 55 | 0.89 | 7 | Unknown |
| 11990232-114 | 5,528 | 12,872 | 0.43 | 6,071 | 9,528 | 0.64 | ND | Unknown |
| 11990232-42 | 171 | 389 | 0.44 | 229 | 316 | 0.72 | 5 | Unknown |
| 11990232-86 | 129 | 289 | 0.45 | 41 | 71 | 0.58 | 7 | Unknown |
| 11990232-82 | 130 | 290 | 0.45 | 125 | 205 | 0.61 | 10 | Unknown |
| 11990232-33 | 158 | 353 | 0.45 | 31 | 39 | 0.81 | 5 | Unknown |
| 11990232-49 | 527 | 1,141 | 0.46 | 358 | 519 | 0.69 | 5 | Unknown |
| 11990232-44 | 92 | 196 | 0.47 | 60 | 85 | 0.71 | 1 | Unknown |
| 11990232-90 | 80 | 170 | 0.47 | 17 | 17 | 1.01 | 9 | Unknown |
| 11990232-43 | 163 | 341 | 0.48 | 248 | 280 | 0.88 | 1 | Unknown |
| 11990232-8 | 415 | 855 | 0.49 | 119 | 256 | 0.47 | ND | Unknown |
| Genes with lower transcript levels in DpDf seedlings | ||||||||
| BG873775 | 468 | 621 | 0.75 | 448 | 938 | 0.48 | 1 | Unknown |
| CD991129 | 898 | 1,097 | 0.82 | 311 | 626 | 0.50 | 6 | Harpin-induced protein 1 family |
Fold ratios that are not statistically significant (t test, P > 0.5) are shown in italics.
An asterisk indicates a gene with a likely qualitative pattern of gene expression changes.
Chromosome positions predicted by BLASTing the maize genome sequence and validated using oat × maize chromosome addition lines (Kynast et al., 2001). ND, Not determined by either method. All of the genes on chromosome 5 in this table are trisomic.