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. 2010 Feb;152(2):927–938. doi: 10.1104/pp.109.150466

Table III.

qRT-PCR analysis of 30 genes differentially expressed in DpDf and wild-type plants in six tissues

Accession Ploidya Fold Ratio (DpDf/Wild-Type Meristem)bcd Fold Ratio (DpDf/Wild-Type Unexpanded Leaves)bcd Fold Ratio (DpDf/Wild-Type Developing Leaves)bcd Fold Ratio (DpDf/Wild-Type Mature Leaves)bcd Fold Ratio (DpDf/Wild-Type Roots)bcd Fold Ratio (DpDf/Wild-Type Immature Ears)bcd
Genes with higher transcript levels in DpDf
    AW289130 Trisomic Turned on Turned on Turned on Turned on Turned on Turned on
    BM380426 Monosomic 7.31 8.62 Turned on Turned on 2.12 4.94
    AI783234 Trisomic 2.38 2.53 1.97 2.03 1.18 Turned on
    BM331974 Trisomic 2.24 2.12 1.97 1.99 Turned on 1.12
    AY105653 Trisomic 2.14 2.32 2.05 1.89 1.19 Turned on
    BM379473 Trisomic 2.12 1.97 Turned on Turned on NE Turned on
    BM332751 Disomic 2.08 NE NE NE NE 3.12
    CF626580 Trisomic 2.08 1.91 1.63 1.12 1.35 Turned on
    BM335301 Disomic 2.05 NE NE NE Turned on 5.16
    CK369759 Trisomic 1.71 4.31 Turned on Turned on Turned on 4.41
    U17897 Trisomic 1.36 1.65 1.97 2.35 NE NE
    AY639019 Trisomic 1.28 3.15 Turned on Turned on Turned on Turned on
    CF629635 Trisomic 1.07 1.53 1.82 2.53 NE Turned on
    L16798 Trisomic 0.95 1.14 Turned on Turned on Turned on Turned on
Genes with lower transcript levels in DpDf
    AY109249 Disomic 0.37 0.44 Turned off Turned off Turned off NE
    11990232-86 Disomic 0.38 Turned off Turned off Turned off 0.83 Turned off
    11990232-44 Disomic 0.45 0.38 Turned off Turned off Turned off NE
    11990232-33 Disomic 0.47 NE NE NE NE 0.34
    11990232-89 Disomic 0.48 0.18 Turned off Turned off 0.98 0.13
    AI622092 Monosomic 0.48 0.45 0.48 Turned off Turned off Turned off
    11990232-90 Disomic 0.50 NE NE NE 0.91 Turned off
    11990232-8 ND 0.52 0.42 0.39 Turned off NE Turned off
    40794996-111 Disomic 0.55 NE NE NE 0.52 Turned off
    BM073880 ND 0.56 0.54 Turned off Turned off 0.36 0.66
    BM379784 Monosomic 0.57 0.62 0.42 Turned off 0.95 Turned off
    AI737202 Monosomic 0.59 0.52 Turned off Turned off Turned off Turned off
    BM331837 ND 0.64 0.47 0.56 0.39 0.89 0.54
    AY107007 Monosomic 0.72 0.22 Turned off Turned off 0.56 0.25
    CD991129 Monosomic 0.79 0.27 0.18 Turned off 0.47 NE
    BM079913 Disomic 0.79 0.61 0.62 0.49 0.85 0.40
a

ND, Not determined.

b

Fold ratios are indicated for genes showing quantitative changes in expression. For genes with detected expression only in DpDf or wild-type plants, the direction of the change is indicated. A gene was defined as turned on when its transcript was absent in a wild-type tissue and present in an aneuploid tissue. Similarly, a gene was defined as turned off when its transcript was present in a wild-type tissue and absent in an aneuploid tissue.

c

Statistically insignificant changes in gene expression (t test, P > 0.5) are indicated in italics.

d

NE, Not expressed. Expression of the gene is not detected in both DpDf and wild-type plants in a particular tissue.