Figure 2.

Sorghum and maize PEPC activity are inhibited by anionic phospholipids. Crude extracts were incubated at 30°C during 30 min in the presence of 50 μm of the phospholipids indicated, and PEPC activity was assayed at pH 8.0 as described in Supplemental Materials and Methods S1. A, Sorghum leaves. B, Maize leaves. C, Arabidopsis leaves. Data are means ± se of three to six different experiments. One-hundred percent activity corresponded to 1 unit mL⁻¹ for sorghum and maize and to 0.1 unit mL⁻¹ for Arabidopsis.