Figure 2.

Nonphosphorylated solubilized maltodextrins inhibit the phosphatase activity of AtSEX4. B-type MD_cryst was prephosphorylated (and prelabeled) for 60 min using StGWD. Equal amounts of AtSEX4 were preincubated with varying amounts of nonphosphorylated B-MD_cryst (A), nonphosphorylated B-MD_sol (B), maltotriose (C), or maltoheptaose (D). Subsequently, equal amounts of the prephosphorylated insoluble MD were added and the orthophosphate released after 5 min was determined as in Figure 1. n = 3; ±sd.