Table II.
Incomplete dephosphorylation of insoluble phosphoglucans using varying levels of AtSEX4
The B-type allomorph of MDcryst, prephosphorylated with StGWD and [β-33P]ATP for 60 min, was incubated with either 25 or 100 ng of AtSEX4. At intervals, the levels of [33P]orthophosphate were determined. [33P]Orthophosphate released is given as percentage of the total 33P content of the prephosphorylated MD. Mean values n = 3; ±sd.
| Time |
33P-Orthophosphate Released |
|
|---|---|---|
| 25 ng SEX4 | 100 ng SEX4 | |
| min | % | |
| 5 | 30 ± 3 | 33 ± 2 |
| 10 | 36 ± 2 | 36 ± 4 |
| 30 | 38 ± 3 | 41 ± 4 |
| 60 | 40 ± 3 | 46 ± 3 |