Table VII.

Simultaneous action of AtSEX4 and StGWD on prelabeled insoluble (phospho)glucans

The B-type allomorph of MD_cryst was prephosphorylated (and prelabeled) for 5 or 60 min using StGWD and [β-³³P]ATP. Subsequently, the insoluble MD were incubated for 60 min with StGWD, AtSEX4, or StGWD plus AtSEX4. At intervals, aliquots were centrifuged and the supernatants were collected. The content of [³³P]orthophosphate, ³³P-phosphoglucans, and of the total glucosyl residues was determined. n.d., Below the limit of detection. Mean values are given (n = 3; ±sd).

	Incubation Time	Preincubation
		5 min	60 min	5 min	60 min	5 min	60 min
		33P-Orthophosphate Released [pmol]		33P in Soluble Phosphoglucans [pmol]		Glc Residues Released [nmol]
	min
StGWD	0	n.d.	n.d.	n.d.	0.8	n.d.	0.5
	10	n.d.	n.d.	0.043	9.2	n.d.	6.1
	20	n.d.	n.d.	0.081	18.0	n.d.	2.2
	30	n.d.	n.d.	0.090	26.4	0.22	44.4
	40	n.d.	n.d.	0.105	27.2	45.5	44.4
	50	n.d.	n.d.	0.189	30.4	46.6	50.0
	60	n.d.	n.d.	0.234	31.2	55.5	55.0
AtSEX4	0	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
	10	0.08	8.4	n.d.	n.d.	n.d.	n.d.
	20	0.16	13.6	n.d.	n.d.	n.d.	n.d.
	30	0.20	17.6	n.d.	n.d.	n.d.	n.d.
	40	0.22	18.4	n.d.	n.d.	n.d.	n.d.
	50	0.22	19.2	n.d	n.d	n.d	n.d
	60	0.23	19.2	n.d.	n.d.	n.d.	n.d.
StGWD + AtSEX4	0	n.d.	n.d.	n.d.	n.d.	n.d.	n.d
	10	0.55	21.6	n.d.	n.d.	n.d.	n.d.
	20	0.75	29.6	n.d.	n.d.	n.d.	n.d.
	30	0.80	32.4	n.d.	n.d.	n.d.	n.d.
	40	0.84	34.0	n.d.	n.d.	0.13	0.33
	50	0.86	35.2	n.d.	n.d.	0.27	0.50
	60	0.86	35.2	n.d.	n.d.	0.27	0.55