Figure 2.

Suc uptake and TaSUT gene expression. A and B, ¹⁴C label incorporation was measured after 10 min of labeling with [¹⁴C]Suc of immature grains of wild-type Certo and lines HOSUT10 (A) and HOSUT20 (B) at 19 DAF. Thirty-minute preincubation was done with the covalent protein modifier diethylpyrocarbonate (DEPC), known to inhibit proton-coupled symport. Significant differences according to t test are as follows: ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. C, Quantitative RT-PCR of gene expression of endogenous wheat Suc transporters in HOSUT10 and wild-type endosperm. Values shown are means of at least three replicates ± sd.