Mutation of gene At1g10310 causes decreased seed 18:1Δ9 fatty acids. A, Schematic representation of the At1g10310 gene model and two insertion alleles. White rectangles represent exons, black rectangles represent 5′ and 3′ untranslated regions, solid lines represent introns and intergenic regions, and gray triangles represent a T-DNA insertion for SALK_125505C and a Ds element for RIKEN line 15-1699-1. B, At1g10310 steady-state mRNA levels in wild-type Columbia (WT Col), a line derived from a wild-type segregant plant (WT Segregant), SALK_125505C (T-DNA), and RIKEN mutant line 15-1699-1 (Ds). C, Seed fatty acid composition of a line derived from a wild-type segregant plant (WT Segregant), the Columbia ecotype (WT Col), and the two mutant lines, T-DNA and Ds, as determined by GC-FID of FAMEs. Error bars represent sd values for five biological replicates (each replicate consisted of 30 seeds). Differences between the mutant and the wild type of greater than 15% that are statistically significant (Student's t test, P < 0.01) compared with the wild type are marked by asterisks.