Figure 2.

35S:At1g10310 mRNA overexpresser lines have more 18:1Δ⁹ and less 18:3 seed fatty acids than the wild type. A, Eighteen-carbon seed fatty acid composition for the wild type (WT Col) and four transgenic lines (35S:line-1 to -4) transformed with a 35S:At1g10310 construct. Each replicate consisted of 30 seeds, and the error bars represent sd values for three biological replicates. Considerable changes (>15%) relative to the wild type that are statistically significant (Student's t test, P < 0.01) are marked by asterisks. B, Semiquantitative PCR shows that the four lines are high expressers of At1g10310 mRNA. Plants were grown under 16-h-light/8-h-dark conditions. [See online article for color version of this figure.]