Figure 6.
Molecular basis of the altered FAME phenotype of mutant SALK_006881C. A, Locations and details of two mutations identified in SALK_006881C on chromosomes (Chr) II and III (centromeres are depicted by black ovals; gene models and mutation sites are schematically represented as described in the Fig. 1 legend). Based on gene model At3g15850.1, the 8-bp deletion (8 bp del; indicated with an arrow) occurs 1,600 bp downstream of the FAD5 translation start site. B, GC-FID chromatogram traces of total leaf FAME extracts of wild-type (WT Col), SALK_006881C, and fad5-1 plants (Heilmann et al., 2004a). Peaks for 16:1Δ7, 16:2, and 16:3 are indicated by arrows. Based upon these results, the FAD5 allele found in SALK_006881C is now designated fad5-2. The insertional mutation in At2g37290 has been renamed rgc1-1.