Figure 4.

S4B staining pattern in prc1-1 root cells. A, Mosaic of micrographs from a 5-d-old light-grown prc1-1 seedling stained with S4B. Arrowheads show multiple bright patches in a single trichoblast. Irregular bright staining along root likely represents dead cells that have absorbed S4B. B, Enlargement of root tip cells showing fibrillar staining. C, Enlargement of lateral root cap cells showing large gaps in thick, curved fibrillar staining (e.g. arrowhead). D, Enlargement of differentiation zone cell showing rupture in trichoblast cell wall (arrowhead). All images are maximum projections of z series. Scale bars are 50 μm (A) or 10 μm (for B–D).