TABLE 2.

Comparison of LAMMER protein kinase consensus phosphorylation sites with a sequence present in all EF1γ orthologs

Position	−7	−6	−5	−4	−3	−2	−1	0	+1	+2	+3
LAMMER kinase consensus	R/D	R/K	R/H	E/R	R	E/R	H/R	S	R	R/E	D/R
		D	E			A	E			D	E
EF1γ consensus	D	D/E	F/Y/W	K	R	—	Y	S	N	E	D

The one-letter amino acid code is used. Boldface letters in the LAMMER kinase consensus sequence indicate amino acids that were most preferentially selected (selectivity value >2.0), while underlined residues are invariant in all identified substrates. Residues are numbered relative to the phosphorylation site at 0, data from Nikolakaki et al. (2002). Boldface letters in the EF1γ consensus indicate identical amino acids present in at least 9 of the 10 arbitrarily selected species compared; underlined residues are invariant. Serine 294 is the principal residue phosphorylated in Drosophila EF1γ by DOA and and PK12 kinases. The 9 arbitrarily chosen species and 11 orthologs compared were: D. melanogaster, Homo sapiens, Oryctolagus cuniculus, Artemia salina, Caenorhabditis elegans, Trypanosoma cruzi, Schizosaccharomyces pombe, and two paralogs each from Xenopus laevis and Saccharomyces cerevisiae.