Figure 2.—

Gene organization and splice variants of Gmtra. (A) Exons in red (E2a, E4, E5, and E6) contain the long ORF that is intact in the female-specific transcripts Gmtra^F1. Exons in blue (E2b, E3a, and E3b) are male specific and contain several in-frame translation termination signals. Black bars indicate the location of small clusters of putative TRA/TRA2 binding sites with the numbers of sites given in parentheses (see Figure S1 for sequences). Arrows above the top line represent the position of primers used for expression analysis. (B) RT–PCR analysis of total RNA extracted from adult XX and XY flies. Results from female (XX) samples are shown amplified with primers 5 and 18B (lane 1) and with primers Gmtra-13 and Gmtra-3B (lane 2). RT–PCR results from male (XY) samples are shown with primers Gmtra-5 and Gmtra-18B (lane 3) and with primers Gmtra-13 and Gmtra-3B (lane 4).