Figure 3.—

Expression patterns of neuronal GAL4 drivers used for neuroanatomical mapping of kcc function. Shown is the fluorescence observed upon excitation at 470 nm when a UAS-mCD8∷GFP reporter is combined with each of the GAL4 drivers indicated. Both elav and 1407 display pan-neuronal expression; the apparent enrichment of elav-GAL4 signal in mushroom bodies is an artifact of the mCD8-based membrane targeting and has been observed previously (Ito et al. 2003). The Rdl driver produces relatively widespread expression in neurons carrying the Rdl GABA_A receptor. The Gad1 and Cha drivers display abundant expression in GABAergic and cholinergic interneurons, respectively (Salvaterra and Kitamoto 2001; Ng et al. 2002)). Three of the other GAL4 drivers produce relatively circumscribed expression patterns (arrowheads): c232 (ellipsoid body; Renn et al. 1999), 104Y (scatterned neurons, including those in the fan-shaped body; Renn et al. 1999) and GH146 (antennal lobe neurons, which project to the mushroom body) (Stocker et al. 1997). The expression pattern for the OK107 driver is shown with those of the other mushroom body drivers in Figure 5B. The representative brains are from 1- to 2-day-old female flies and are positioned such that the anterior side is facing the camera; dorsal is up and ventral down.