Figure 5.—

Loss of dMyc in wing discs slows their growth and patterning. dMyc (red) and Wg (blue) antibody staining and VgM-GFP expression (green) in 96-hr and late-L3 wing discs. (A) Ninety-six-hour yw;VgM;+ wing disc: (A′) dMyc, (A″) Wg, and (A″′) GFP; (B) 114-hr (late-L3) yw;VgM;+ wing disc: (B′) dMyc, (B″) Wg, and (B″′) GFP; (C) 96-hr dm⁴;VgM; Tub-Gal4/+ wing disc: (C′) dMyc and (C″) Wg expression in 96-hr dm⁴;VgM; Tub-Gal4/+ wing discs (immature relative to dm⁴; Tub-dmyc/+ wing discs of the same chronological age; compare to E″). The pattern in C″ is instead comparable to control yw wing discs at 72 hr AEL (Figure S1, A″–C″); (C″′) GFP; (D) 137-hr (late-L3) dm⁴;VgM;Tub-Gal4/+ wing disc: (D′) dMyc and (D″). By late-L3, Wg expression has matured (compare to B″): (D″′) GFP; (E) 96-hr AEL dm⁴;Tub-dmyc/+ wing disc: (E′) dMyc and (E″) Wg expression for comparison with discs in C–C″.