Figure 4.

Effect of NEM on LHCII protein phosphorylation in salt-washed thylakoid membranes isolated from high-light-treated pumpkin leaves. Isolated thylakoids were washed with assay buffer (H₂O) or with 1 m NaCl in buffer with or without 0.05% (w/v) Triton, and the washed membranes were phosphorylated in vitro in white light in the presence or absence of NEM. Phosphoproteins were detected as described in Figure 1.