TABLE 3.

HKA Tests

Gene	Population	Chromosome	L	n	S	Div	χ2	P-value
Winters SR data
Dox	Nicewicz	X	5521	12	19	0.0567	52.51	<0.0001
Dox	Tremont	X	5956	34	12	0.0567	93.27	<0.0001
Dox	Winters	X	6061	12	8	0.0567	72.17	<0.0001
MDox	Nicewicz	X	4401	12	12	0.0611	41.22b	<0.0001
MDox	Tremont	X	4507	33	9	0.0611	59.31	<0.0001
MDox	Winters	X	4508	12	1	0.0611	49.72	<0.0001
Nmy	Nicewicz	3R	7461	24	0	0.0516	77.34	<0.0001
Nmy	Tremont	3R	7460	65	0	0.0516	94.52	<0.0001
Nmy	Winters	3R	7461	11	1	0.0516	59.92	<0.0001
NmyAlla	Tremont	3R	5403	66	60	0.0513	42.85	<0.0001
NmyAll	Winters	3R	5385	12	121	0.0511	20.28	0.0903
Begun and Whitley (2000) data
bnb	Winters	X	1015	8	11	0.0197	17.99	0.0764
mei-218	Winters	X	1187	8	14	0.0687
ovo	Winters	X	1356	8	9	0.0270
sn	Winters	X	1437	8	28	0.0370
sog	Winters	X	1233	8	8	0.0251
X	Winters	X	1425	8	24	0.0281
yp3	Winters	X	1227	8	8	0.0473
AP-50	Winters	3R	1398	8	58	0.0293
fzo	Winters	3R	1360	8	22	0.0708
hyd	Winters	3R	1786	8	26	0.0208
Osbp	Winters	3R	1166	8	31	0.0266
ry	Winters	3R	1362	8	54	0.0419
T-cp1	Winters	3R	1201	8	9	0.0325

L, number of bases sequenced in D. simulans; n, number of D. simulans chromosomes sampled; S, number of segregating sites; Div, per-base divergence from D. melanogaster; χ² and P-values correspond to multilocus HKA tests on 13 loci previously sequenced in D. simulans (bottom) and when data from single Winters SR genes for each North American population were added to the 13 loci (top). See text for details.

^aAncestral alleles were not excluded from the analysis (Nicewicz has no ancestral alleles in sample).