TABLE 2.
Quantification of the number of Mst77Y genes and their mRNA expression through XbaI digestion
| Template for PCR | Replica | Mass 179-bp band | Mass 110-bp band | Mst77Y/Mst77F ratioa | Mst77Y genes | % Mst77Y mRNAb |
|---|---|---|---|---|---|---|
| gDNA | 1 | 6.71 | 35.04 | 8.50 | 17.0 | — |
| gDNA | 2 | 6.75 | 32.67 | 7.88 | 15.8 | — |
| gDNA | 3 | 6.72 | 33.14 | 8.02 | 16.0 | — |
| Mean (95% C.I.)c | 16.3 (14.7–17.9) | |||||
| cDNA | 1 | 36.58 | 8.59 | 0.38 | — | 27.6 |
| cDNA | 2 | 33.20 | 8.46 | 0.41 | — | 29.3 |
| cDNA | 3 | 35.09 | 8.23 | 0.38 | — | 27.6 |
| Mean (95% C.I.)c |
28.2 (25.8–30.6) |
a
Number of Mst77Y molecules in relation to Mst77F, estimated as (column 4/column 3) × (179/110).
b
Contribution of Mst77Y genes to the Mst77F-like mRNA pool, estimated as column 5/(1 + column 5).
c
Confidence intervals are rough approximations, assuming normal distribution.