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SPT3 is a negative regulator of GCN5. Deleting SPT3 rescues the 3-AT hypersensitivity (A) and HIS3 transcriptional defects (B, shown are RT–PCR results). (C) gcn5 TSTY/4A suppresses the gal− phenotype caused by SPT3 deletion. Yeast cells were serially diluted and spotted to glucose (Glc) or galactose (Gal) medium.
