Table II.
Cre::Vir protein translocation from A. tumefaciens into Arabidopsisa
| Total No. Kmr Calli/No. Root Explantsc
|
Transfer Efficiencyd
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Strainb | Plasmidb | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
| No. kmr Calli/100 Expl | |||||||||
| LBA1100 | Cre | 1/500 | 0/500 | ND | 0/515 | 0.2 ± 0.3 | 0 | - | 0 |
| Cre::VirFΔ42N | 338/350 | 352/600 | 352/580 | 612/515 | 97 ± 3 | 58 ± 2 | 60 ± 4 | 120 ± 18 | |
| Cre::VirE2 | 87/475 | 88/460 | 22/530 | 157/515 | 18 ± 3 | 19 ± 6 | 4 ± 0.3 | 31 ± 2 | |
| Cre::VirE2-50C | ND | 20/480 | 2/600 | 10/280 | - | 4 ± 0.3 | 0.3 ± 0.5 | 4e | |
| Cre::VirE3-50C | ND | 21/540 | 13/580 | 124/525 | - | 4 ± 0.5 | 2 ± 1 | 24 ± 4 | |
| Cre::VirE3 | ND | ND | 0/500 | 10/800 | - | - | - | 1 ± 0.7 | |
| LBA2571 | Cre::VirFΔ42N | 200/345 | 388/620 | ND | 390/530 | 57 ± 16 | 63 ± 18 | - | 74 ± 9 |
| Cre::VirE2 | 42/420 | 42/565 | 14/450 | 157/480 | 10 ± 3 | 7 ± 1 | 3 ± 1 | 33 ± 5 | |
| Cre::VirE2-50C | Inf | 15/600 | 0/520 | 6/310 | - | 2 ± 1 | 0 | 2e | |
| Cre::VirE3-50C | 24/445 | 22/495 | 2/555 | 99/590 | 5 ± 0.9 | 4 ± 0.3 | 0.3 ± 0.01 | 17 ± 2 | |
a Arabidopsis C24, line 3043 contains a lox-substrate for detection of in planta Cre activity, detected as kanamycin resistance. A. tumefaciens was cocultivated with root explants for 3 d. Kmr calli were scored 3 weeks after cocultivation. In four independent experiments, two petri dishes per strain/plasmid combination were assayed.
b A. tumefaciens strain LBA1100 (wild-type vir) or virE1 mutant LBA2571 carrying a non-mobilizable plasmid expressing Cre alone, Cre::VirFΔ42N, Cre::VirE2, Cre::VirE2-50C, Cre::VirE3-50C, or Cre::VirE3, respectively.
c Total no. of calli and root explants per two petri dishes. ND, Not determined.
d Transport efficiency presented as no. of Kmr calli per 100 root explants (± sd).
e Nos. per single petri dish due to infection.