Table. Transmission of cattle BSE, sheep BSE, and sheep scrapie isolates in transgenic mice expressing porcine prion protein*.
| TSE isolate | Genotype | PrPres, kDa | First passage |
Second passage |
Third passage |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Survival time, dpi | % (Attack rate) | Survival time, dpi | % (Attack rate) | Survival time, dpi | % (Attack rate) | |||||
| Cattle-BSE1 | – | 19 | >650 | 0 (0/12)† | 269 ± 3 | 100 (10/10) | 204 ± 12 | 100 (9/9) | ||
| Cattle-BSE2 | – | 19 | 498 ± 9 | 17 (2/12)† | 198 ± 6 | 100 (15/15) | 193 ± 17 | 100 (6/6) | ||
| Cattle-BSE0 | – | 19 | >650 | 19 (3/16)† | 197 ± 4 | 100 (12/12) | 190 ± 10 | 100 (7/7) | ||
| Sheep-BSE0 | ARQ/ARQ | 19 | 458 ± 11 | 100 (15/15) | 162 ± 4 | 100 (13/13) | 166 ± 7 | 100 (7/7) | ||
| SC-662/97 | ARQ/ARQ | 21 | >650 | 0 (0/10) | >650 | 0 (0/12) | ND | ND | ||
| SC-UCD/99 | ARQ/ARQ | 21 | >650 | 0 (0/12) | >650 | 0 (0/9) | ND | ND | ||
| SC-Langlade | ARQ/ARQ | 21 | >650 | 0 (0/12) | >650 | 0 (0/12) | ND | ND | ||
| SC-PS13 | ARQ/ARQ | 20 | >650 | 0 (0/12) | >650 | 0 (0/12) | ND | ND | ||
| SC-JR01 | VRQ/VRQ | 21 | >650 | 0 (0/12) | >650 | 0 (0/12) | ND | ND | ||
| SC-PS83 | ARR/ARR | 21 | >650 | 0 (0/12) | >650 | 0 (0/12) | ND | ND | ||
| SC-PS48 | VRQ/VRQ | 19 | >650 | 0 (0/9) | >650 | 0 (0/10) | ND | ND | ||
| SC-PS152 | AfRQ/AfRQ | ≈7–14 | 300–600 | 16 (2/12) | 162 ± 13 | 100 (9/9) | 172 ± 16 | 100% (7/7) | ||
| SC-UCD/99 adapted to BoPrP-Tg110 | –- | >650 | 0 (0/13) | >650 | 0 (0/10) | ND | ND | |||
| Healthy sheep brain | ARQ/ARQ | >650 | 0 (0/14) | >650 | 0 (0/9) | ND | ND | |||
*BSE, bovine spongiform encephalopathy; TSE, transmissible spongiform encephalopathy; PrPres, atypical proteinase K–resistant prion protein; dpi, days postinoculation; ND, not determined. †No. animals positive for the aberrant form of PrP associated with disease / no. inoculated animals.