Table II.
Oligonucleotides
List of oligonucleotides used for plasmid-specific PCRS and attR mutagenesis in this study.
| Oligonucleotides | Sequence |
|---|---|
| pAtC58 primers | |
| attDa | 5′CGGGGTACCCCGATGCACAATGGATCAACG3′b |
| 5′CCC AAG CTT GGG TTA GAT AGT TTG TGA GCC3′c | |
| attR (full length)a | 5′CGGGGTACCCCGATTATAAAAACCATTCAT3′b |
| 5′CCCAAGCTTGGGCTAGGCGATTTTGAGAGC3′c | |
| attR (502 bp) | 5′TGACGCAATCCATCCTATCA3′ |
| 5′AGCGGGATAACGCATTCTAA3′ | |
| Primers to amplify attR internal fragment for mutagenesis | 5′ATTCATGGCAGCGATAAAGG3′ |
| 5′AGCGGGATAACGCATTCTAA3′ | |
| attR-lacZ primers to screen insertions in the attR gene | 5′CGGGGTACCCCGATTATAAAAACCATTCAT3′ |
| 5′CCGCCACATATCCTGATCTT3′ | |
| attH1 | 5′CGGTTTCAAGATCGAGTGGT3′ |
| 5′ATAACCTTGTCCCGATGTGC3′ | |
| pTiC58 | |
| virB9 | 5′GCATTTCTCACTCTGGCATGT3′ |
| 5′GATCGTAGGCGGTATTCCAA3′ |
a Primers originally designed for cloning.
b Underlined portion indicates KpnI site.
c Underlined portion indicates HindIII site.