Table 2. Geometric mean titers and percentage coefficient of variations of absolute titers and titers relative to candidate antibody standard 07/150 for each serum sample for each influenza virus by hHI and neutralization*†.
| Serum sample | Virus assay, antigen, and clade |
||||||
|---|---|---|---|---|---|---|---|
| hHI (GMT, %GCV, %GCV relative to 07/150) |
Neutralization (GMT, %GCV, %GCV relative to 07/150) |
||||||
| NIBRG-14 clade 1 | NIBRG-23 clade 2.2 | IBCDC-RG5 clade 2.3 | NIBRG-14 clade 1 | NIBRG-23 clade 2.2 | IBCDC-RG5 clade 2.3 | ||
| Prevaccination | |||||||
| B | 7, 110, 72 | 7, 111, 86 | 6, 112, 85 | 12, 176, 202 | 12, 141, 173 | 10, 68, 223 | |
| N | 6, 31, 46 | 5, 29, 18 | 5, 22, 0 | 14, 175, 228 | 13, 160, 215 | 10, 186, 250 | |
| J |
10, 111, 123 |
8, 81, 155 |
6, 43, 96 |
|
19, 175, 228 |
18, 218, 309 |
11, 102, 287 |
| Postvaccination | |||||||
| Low | |||||||
| C | 34, 84, 36 | 17, 29, 18 | 10, 126, 164 | 63, 183, 80 | 27, 201, 236 | 23, 122, 206 | |
| D | 15, 141, 105 | 8, 110, 127 | 6, 68, 45 | 19, 232, 248 | 15, 215, 288 | 12, 98, 226 | |
| High | |||||||
| E | 104, 133, 58 | 60, 96, 36 | 8, 128, 141 | 148, 191, 81 | 87, 159, 42 | 20, 118, 223 | |
| F | 78, 97, 55 | 16, 141, 116 | 20, 104, 105 | 83, 144, 62 | 18, 152, 234 | 35, 147, 196 | |
| G | 281, 152, 61 | 147, 102, 131 | 44, 163, 144 | 504, 132, 37 | 274, 217, 83 | 140, 103, 45 | |
| H | 95, 118, 66 | 42, 144, 116 | 18, 106, 111 | 130, 166, 74 | 91, 157, 33 | 34, 127, 154 | |
| I | 351, 125, 60 | 93, 91, 47 | 107, 75, 44 | 379, 199, 71 | 106, 207, 191 | 161, 136, 41 | |
| A | 391, 138, 55 | 335, 114, 34 | 448, 119, 51 | 1,389, 86, 45 | 1,313,125, 76 | 2,893, 78, 63 | |
| L |
391, 147, 61 |
398, 145, 42 |
480, 108, 44 |
|
1,453, 104, 63 |
1,520, 101, 85 |
3,097, 73, 52 |
| False positive (K) |
37, 120, 34 |
24, 136, 135 |
8, 81, 117 |
|
52, 185, 143 |
44, 185, 53 |
13, 112, 298 |
| Sheep | |||||||
| O | 48, 262, 285 | 30, 245, 338 | 17, 147, 319 | 216, 139, 53 | 148, 170, 33 | 49, 117, 156 | |
| P | 1,857, 582, 535 | 1,171, 496, 487 | 1,338, 487, 545 | 7,317, 148, 196 | 732, 283, 144 | 3,806, 145, 59 | |
*GMT geometric mean titer; %GCV, percentage geometric coefficient of variation; hHI, hemagglutination-inhibition assay using horse erythrocytes; neutralization, virus neutralization assay. (Paired samples were B and C, N and F, and J and H.) †Overall serum samples, GMT by neutralization vs. hHI (p = 0.001, NIBRG-14; p = 0.008, NIBRG-23; p = 0.001, IBCDC-RG5; Wilcoxon signed-rank test).