Table II.
Frequency of site-specific T-DNA integration
| Experimental Step | No. of Plants | Frequency of Transformation Events % of Total |
|---|---|---|
| Hygromycin | 620 | 100 |
| 5-FC-resistant plants | 82 | 13.22 |
| Plants analyzed by PCR | 68 | 10.96 |
| PCR fragment > 600 bp or no PCR fragment | 23 | 3.71 |
| All cases of T-DNA integration | 16 | 2.58 |
| Integration of I-SceIORF T-DNA | 4 | 0.64 |
| Integration of full-length or slightly truncated T-DNA | 5 | 0.81 |
| Integration of I-SceI-digested uidA-hpt T-DNA | 7 | 1.13 |
| Large deletion/insertion | 7 | 1.13 |
| PCR fragment < 600 bp | 45 | 7.25 |