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Strategy to detect the effects of kinase inhibitors on the cellular signaling network. A, three SILAC-labeled cell populations were subjected to no treatment, treatment with growth factor, and treatment with growth factor in the presence of kinase inhibitor. B, principal response of the targeted kinase and its downstream substrates in the three SILAC conditions. C, three major response patterns reflected in SILAC mass spectra. EGFR, EGF receptor.
