Table I. Identified peptides and assigned spectra (in parentheses) of the CAP after cAMP-CC capturing in E. coli lysate (500 μg of total protein).
8-ADOA-cAMP-CC (7d) | 8-AHA-cAMP-CC (7c) | 8-ABA-cAMP-CC (7b) | 8-AEA-cAMP-CC (7a) | 6-AH-cAMP-CC (5b) | 6-AE-cAMP-CC (5a) | 2-AHA-cAMP-CC (6b) | 2-AEA-cAMP-CC (6a) | 8-AHA-cAMP Cmptna | |
---|---|---|---|---|---|---|---|---|---|
Off bead | 7 (7) | 9 (24) | 10 (13) | 1 (1) | 11 (46) | 10 (20) | 11 (44) | 8 (9) | 2 (2) |
On bead | 12 (25) | 13 (56) | 13 (27) | 5 (5) | 12 (58) | 12 (16) | 11 (87) | 13 (60) | 6 (9) |
a 8-AHA-cAMP Cmptn refers to competition experiments using Capture Compound 7c in the presence of 2 mm 8-AHA-cAMP (4c).