Fig. 2.

VI16743 affinity matrix permits comprehensive one-step purification of human protein kinases. A, evaluation of protein distribution and Mascot identification score in VI16743-purified fractions demonstrate the high selectivity of the immobilized inhibitor for protein kinases. Error bars indicate the standard deviation within four independent experiments. B, VI16743 enables purification of protein kinases from nearly all groups of the human kinome. aPK, atypical protein kinases; PK, protein kinases; CAMK, calcium/calmodulin-dependent kinases; TK, tyrosine kinases; TKL, tyrosine kinase-like kinases. STE, sterile homologue kinases; AGC, PKA/PKG/PKC-family kinases; RCG, receptor guanylate cyclases; CMGC, CDK/MAPK/GSK3/CLK family kinases.