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. 2003 Nov;133(3):1072–1082. doi: 10.1104/pp.103.025999

Figure 1.

Figure 1.

AvrRpt2 can be cleaved in rabbit reticulocyte lysate. a, Smaller products observed are cleaved products of AvrRpt2. AvrRpt2 (lanes 1–3), GST::AvrRpt2 (lanes 4–8), and AvrB (lanes 9–11) were translated and 35S-labeled using rabbit reticulocyte lysate. The durations of incubations are indicated. In lanes 7 and 8, EDTA was added to a final concentration of 20 mm after a 0.5-h in vitro translation reaction to stop the reaction. The hours indicated are the total of the reaction time and the incubation time following the EDTA addition. The labeled proteins were resolved by denaturing PAGE and were detected by phosphor imaging of the gel. b, AvrRpt2 is cleaved at the correct site in rabbit reticulocyte lysate. The cleaved products of the GST::AvrRpt2 reaction (lane 2) were compared with GST::ΔC72 (lane 1), which corresponds to the N-terminal region expected if the cleavage occurs between G71 and G72, and with ΔN71 (lane 3), which corresponds to the expected C-terminal region. White triangle, Full-length; black triangle, C-terminal region after cleavage; white circle, N-terminal region after cleavage. The N-terminal region was not observed with AvrRpt2 (a, lanes 1–3) due to its small size. The positions of molecular markers are indicated on the right.